The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells. Its affinity to the mouse PD1 protein is   <20 nM.

Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the PD-1 cDNA followed by the PD-1-Ig fusion protein.
Clone: 29F.1A12.1, the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype: rat IgG2a, kappa.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
3 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.