WB
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MCF7 cell lysate (low expression), Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: U-87 MG cell lysate treated with deglycosylation, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with Rabbit anti-TFPI2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TFPI2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input), Lane 2:Rabbit anti-TFPI2 antibody IP in U-87 MG cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TFPI2 antibody in U-87 MG cell lysate, Blocking/Dilution buffer: primary antibody dilution. Exposure time: 3 minutes.| Product Name | TFPI2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human TFPI2 aa 1-235. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TFPI2 |
|---|---|
| Gene Synonyms | PP5 REF1 TFPI-2 |
| Gene Full Name | tissue factor pathway inhibitor 2 |
| Gene Summary | This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | Placental protein 5 antibody PP5 antibody REF1 antibody Retinal pigment epithelium cell factor 1 antibody TFPI 2 antibody TFPI-2 antibody TFPI2 antibody TFPI2 PEN antibody TFPI2_HUMAN antibody Tissue factor pathway inhibitor 2 antibody |
| Molecular Weight(MW) | 27kDa(Observed band size: 30kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody at 1/5,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MCF7 cell lysate (low expression), Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: U-87 MG cell lysate treated with deglycosylation, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with Rabbit anti-TFPI2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TFPI2 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input), Lane 2:Rabbit anti-TFPI2 antibody IP in U-87 MG cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TFPI2 antibody in U-87 MG cell lysate, Blocking/Dilution buffer: primary antibody dilution. Exposure time: 3 minutes.| Application Notes | WB:1:5000 IHC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
