WB
Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody at 1/1000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MCF7 cell lysate (negative), Lane 3: SK-MEL-28 cell lysate, Lane 4: PANC-1 cell lysate (low expression), Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | SLUG Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SLUG aa 1-150. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SNAI2 |
|---|---|
| Gene Synonyms | SLUG WS2D SLUGH SLUGH1 SNAIL2 |
| Gene Full Name | snail family transcriptional repressor 2 |
| Gene Summary | This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008] |
| Alternative Names | MGC10182 antibody Neural crest transcription factor Slug antibody Protein snail homolog 2 antibody Slug (chicken homolog) zinc finger protein antibody Slug homolog zinc finger protein antibody Slug zinc finger protein antibody SLUGH antibody SLUGH 1 antibody SLUGH1 antibody SNAI 2 antibody MGC10182 antibody Neural crest transcription factor Slug antibody Protein snail homolog 2 antibody Slug (chicken homolog) zinc finger protein antibody Slug homolog zinc finger protein antibody Slug zinc finger protein antibody SLUGH antibody SLUGH 1 antibody SLUGH1 antibody SNAI 2 antibody Snai2 antibody SNAI2_HUMAN antibody Snail 2 antibody Snail homolog 2 antibody Snail2 antibody WS 2D antibody WS2D antibody Zinc finger protein SLUG antibody Zinc finger protein SNAI2 antibody |
| Molecular Weight(MW) | 30kDa(Observed band size: 35kDa) |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody at 1/1000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: MCF7 cell lysate (negative), Lane 3: SK-MEL-28 cell lysate, Lane 4: PANC-1 cell lysate (low expression), Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.Glycerol. Preservative: 0.05% Sodium Azide. |
