WB
Western blot analysis of Estrogen Inducible Protein pS2 on different lysates with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/5000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane), Lane 2: MDA-MB-231 cell lysate (negative) (15 µg/Lane), Lane 3: Human liver tissue lysate (negative) (20 µg/Lane), Lane 4: Human kidney tissue lysate (negative) (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of MCF7 (positive) and MDA-MB-231 (negative) labeling Estrogen Inducible Protein pS2 with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of MDA-MB-231 (left, negative) and MCF7 (right, positive) cells labeling Estrogen Inducible Protein pS2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Estrogen Inducible Protein pS2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Estrogen Inducible Protein pS2 aa 35-84 / 84. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000 ICC/IF:1:50-1:200 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TFF1 |
|---|---|
| Gene Synonyms | pS2 BCEI HPS2 HP1.A pNR-2 D21S21 |
| Gene Full Name | trefoil factor 1 |
| Gene Summary | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
| Alternative Names | BCEI antibody Breast cancer estrogen inducible protein antibody Breast cancer estrogen inducible sequence antibody Breast cancer estrogen-inducible protein antibody D21S21 antibody Gastrointestinal trefoil protein antibody Gastrointestinal trefoil protein pS2 antibody hP1.A antibody HP1A antibody HPS 2 antibody BCEI antibody Breast cancer estrogen inducible protein antibody Breast cancer estrogen inducible sequence antibody Breast cancer estrogen-inducible protein antibody D21S21 antibody Gastrointestinal trefoil protein antibody Gastrointestinal trefoil protein pS2 antibody hP1.A antibody HP1A antibody HPS 2 antibody HPS2 antibody pNR 2 antibody PNR-2 antibody pNR2 antibody Polypeptide P1.A antibody Protein pS2 antibody PS 2 antibody pS2 antibody pS2 protein antibody TFF 1 antibody TFF1 antibody TFF1_HUMAN antibody Trefoil factor 1 antibody |
| Molecular Weight(MW) | 9kDa(Observed band size: 9kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Estrogen Inducible Protein pS2 on different lysates with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/5000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane), Lane 2: MDA-MB-231 cell lysate (negative) (15 µg/Lane), Lane 3: Human liver tissue lysate (negative) (20 µg/Lane), Lane 4: Human kidney tissue lysate (negative) (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of MCF7 (positive) and MDA-MB-231 (negative) labeling Estrogen Inducible Protein pS2 with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Estrogen Inducible Protein pS2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of MDA-MB-231 (left, negative) and MCF7 (right, positive) cells labeling Estrogen Inducible Protein pS2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 ICC/IF:1:50-1:200 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
