WB
Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody at 1/1000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane), Lane 2: K-562 cell lysate (20 µg/Lane), Lane 3: Human placenta tissue lysate (40 µg/Lane), Lane 4: Mouse brain tissue lysate (40 µg/Lane), Lane 5: Rat brain tissue lysate (40 µg/Lane), Lane 6: Rat testis tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling MSI2 with Rabbit anti-MSI2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (i594) was used as the secondary antibody at 1/1,000 dilution.IHC
mmunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MSI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
MSI2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-MSI2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MSI2 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1:HeLa cell lysate (input), Lane 2:Rabbit anti-MSI2 antibody IP in HeLa cell lysate, Lane 3:Rabbit IgG instead of Rabbit anti-MSI2 antibody in HeLa cell lysate, Blocking/Dilution buffer:5% NFDM/TBST, Exposure time:12 seconds.| Product Name | MSI2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MSI2 aa 1-185 / 328. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000 ICC/IF:1:100 IHC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MSI2 |
|---|---|
| Gene Synonyms | MSI2H |
| Gene Full Name | musashi RNA binding protein 2 |
| Gene Summary | This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is transcriptional regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. [provided by RefSeq, Apr 2016] |
| Alternative Names | FLJ36569 antibody MGC3245 antibody Msi2 antibody MSI2/HOXA9 fusion gene included antibody MSI2H antibody MSI2H_HUMAN antibody Musashi 2 antibody Musashi homolog 2 antibody Musashi RNA binding protein 2 antibody Musashi Drosophila homolog of 2 antibody FLJ36569 antibody MGC3245 antibody Msi2 antibody MSI2/HOXA9 fusion gene included antibody MSI2H antibody MSI2H_HUMAN antibody Musashi 2 antibody Musashi homolog 2 antibody Musashi RNA binding protein 2 antibody Musashi Drosophila homolog of 2 antibody Musashi-2 antibody RNA binding protein Musashi homolog 2 antibody RNA-binding protein Musashi homolog 2 antibody WD 40 repeat protein MSI2 antibody |
| Molecular Weight(MW) | 35kDa(Observed band size:35/37kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody at 1/1000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane), Lane 2: K-562 cell lysate (20 µg/Lane), Lane 3: Human placenta tissue lysate (40 µg/Lane), Lane 4: Mouse brain tissue lysate (40 µg/Lane), Lane 5: Rat brain tissue lysate (40 µg/Lane), Lane 6: Rat testis tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling MSI2 with Rabbit anti-MSI2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (i594) was used as the secondary antibody at 1/1,000 dilution.
IHC
mmunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MSI2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
MSI2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-MSI2 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-MSI2 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1:HeLa cell lysate (input), Lane 2:Rabbit anti-MSI2 antibody IP in HeLa cell lysate, Lane 3:Rabbit IgG instead of Rabbit anti-MSI2 antibody in HeLa cell lysate, Blocking/Dilution buffer:5% NFDM/TBST, Exposure time:12 seconds.| Application Notes | WB:1:1000 ICC/IF:1:100 IHC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
