WB
Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody at 1/2000 dilution. Lane 1: A549 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: NCI-H1299 cell lysate, Lane 4: PANC-1 cell lysate, Lane 5: COS-1 cell lysate, Lane 6: C6 cell lysate, Lane 7: Rat lung tissue lysate, Lane 8: Rat kidney tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Integrin beta 5 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Integrin beta 5 aa 1-719. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ITGB5 |
|---|---|
| Gene Full Name | integrin subunit beta 5 |
| Gene Summary | This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin. [provided by RefSeq, Aug 2017] |
| Alternative Names | FLJ26658 antibody Integrin beta-5 antibody Integrin subunit beta 5 antibody integrin beta 5 antibody ITB5_HUMAN antibody Itgb5 antibody Testis secretory sperm-binding protein Li 217p antibody |
| Molecular Weight(MW) | 88kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody at 1/2000 dilution. Lane 1: A549 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: NCI-H1299 cell lysate, Lane 4: PANC-1 cell lysate, Lane 5: COS-1 cell lysate, Lane 6: C6 cell lysate, Lane 7: Rat lung tissue lysate, Lane 8: Rat kidney tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
