WB
Western blot analysis of CD20 on different lysates with Rabbit anti-CD20 antibody at 1/2000 dilution. Lane 1: Raji cell lysate, Lane 2: 293T cell lysate (negative), Lane 3: Ramos cell lysate, Lane 4: HeLa cell lysate (negative), Lane 5: Daudi cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD20 with Rabbit anti-CD20 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD20 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD20 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,=000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
CD20 was immunoprecipitated from 0.2 mg Raji cell lysate with Rabbit anti-CD20 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-CD20 antibody at 1/2,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/10000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input), Lane 2: Rabbit anti-CD20 antibody IP in Raji cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CD20 antibody in Raji cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 59 seconds.| Product Name | CD20 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000 ICC/IF:1:500 IHC:1:1000-1:2000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MS4A1 |
|---|---|
| Gene Synonyms | B1 S7 Bp35 CD20 FMC7 CVID5 LEU-16 |
| Gene Full Name | membrane spanning 4-domains A1 |
| Gene Summary | This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | APY antibody ATOPY antibody B lymphocyte antigen CD20 antibody B Lymphocyte Cell Surface Antigen B1 antibody B-lymphocyte antigen CD20 antibody B-lymphocyte cell-surface antigen B1 antibody B-lymphocyte surface antigen B1 antibody B1 antibody Bp 35 antibody Bp35 antibody APY antibody ATOPY antibody B lymphocyte antigen CD20 antibody B Lymphocyte Cell Surface Antigen B1 antibody B-lymphocyte antigen CD20 antibody B-lymphocyte cell-surface antigen B1 antibody B-lymphocyte surface antigen B1 antibody B1 antibody Bp 35 antibody Bp35 antibody CD 20 antibody CD20 antibody CD20 antigen antibody CD20 receptor antibody CD20_HUMAN antibody CVID 5 antibody CVID5 antibody Fc epsilon receptor I beta chain antibody Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody FCER1B antibody High affinity immunoglobulin epsilon receptor subunit beta antibody IgE Fc receptor subunit beta antibody IGEL antibody IGER antibody IGHER antibody LEU 16 antibody Leu-16 antibody LEU16 antibody leukocyte surface antigen Leu 16 antibody Leukocyte surface antigen Leu-16 antibody Ly44 antibody Membrane spanning 4 domains A1 antibody Membrane spanning 4 domains subfamily A member 2 antibody membrane-spanning 4-domains A1 antibody Membrane-spanning 4-domains subfamily A member 1 antibody MGC3969 antibody MS4A1 antibody MS4A2 antibody S7 antibody |
| Molecular Weight(MW) | 33kDa(Observed band size:33kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD20 on different lysates with Rabbit anti-CD20 antibody at 1/2000 dilution. Lane 1: Raji cell lysate, Lane 2: 293T cell lysate (negative), Lane 3: Ramos cell lysate, Lane 4: HeLa cell lysate (negative), Lane 5: Daudi cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Raji (positive) and 293T (negative) labeling CD20 with Rabbit anti-CD20 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD20 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD20 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,=000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
CD20 was immunoprecipitated from 0.2 mg Raji cell lysate with Rabbit anti-CD20 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-CD20 antibody at 1/2,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/10000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input), Lane 2: Rabbit anti-CD20 antibody IP in Raji cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CD20 antibody in Raji cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 59 seconds.| Application Notes | WB:1:2000 ICC/IF:1:500 IHC:1:1000-1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
