WB
Western blot analysis of IgA on human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | IgA Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant full length protein of Human IgA aa 1-353 / 353. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGHA1 |
|---|---|
| Gene Synonyms | IgA1 |
| Gene Full Name | immunoglobulin heavy constant alpha 1 |
| Gene Summary | Contributes to immunoglobulin receptor binding activity. Involved in glomerular filtration and positive regulation of respiratory burst. Located in extracellular space. Part of monomeric IgA immunoglobulin complex and secretory dimeric IgA immunoglobulin complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Hepatocellular carcinoma-associated protein TB6 antibody Ig alpha 1 chain C region antibody Ig alpha 2 chain C region antibody IGHA antibody IGHA1 antibody IGHA2 antibody Immunoglobulin heavy constant alpha 1 antibody Immunoglobulin heavy constant alpha 2 A2m marker antibody Immunoglobulin heavy constant alpha 2 antibody PIgR antibody Hepatocellular carcinoma-associated protein TB6 antibody Ig alpha 1 chain C region antibody Ig alpha 2 chain C region antibody IGHA antibody IGHA1 antibody IGHA2 antibody Immunoglobulin heavy constant alpha 1 antibody Immunoglobulin heavy constant alpha 2 A2m marker antibody Immunoglobulin heavy constant alpha 2 antibody PIgR antibody Poly-Ig receptor antibody polymeric immunoglobulin receptor antibody polymeric immunoglobulin receptor Secretory component antibody |
| Molecular Weight(MW) | 38kDa(Observed band size: 60kDa) |
| Cellular Localization | Cell membrane, Secreted. |
WB
Western blot analysis of IgA on human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
