WB
Western blot analysis of MFGE8 on different lysates with Rabbit anti-MFGE8 antibody at 1/1000 dilution. Lane 1: A549 cell lysate, Lane 2: A431 cell lysate, Lane 3: HeLa cell lysate, Lane 4: MCF7 cell lysate, Lane 5: SK-Br-3 cell lysate, Lane 6: BT-20 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Product Name | MFGE8 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within human MFGE8 aa 1-387. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MFGE8 |
|---|---|
| Gene Synonyms | BA46 HMFG MFGM SED1 hP47 EDIL1 MFG-E8 SPAG10 OAcGD3S HsT19888 |
| Gene Full Name | milk fat globule EGF and factor V/VIII domain containing |
| Gene Summary | This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Alternative Names | BA46 antibody Breast epithelial antigen BA46 antibody EDIL1 antibody HMFG antibody hP47 antibody HsT19888 antibody Lactadherin antibody Medin antibody MFG-E8 antibody MFGE8 antibody BA46 antibody Breast epithelial antigen BA46 antibody EDIL1 antibody HMFG antibody hP47 antibody HsT19888 antibody Lactadherin antibody Medin antibody MFG-E8 antibody MFGE8 antibody MFGM antibody MFGM_HUMAN antibody Milk fat globule EGF factor 8 antibody Milk fat globule EGF factor 8 protein antibody Milk fat globule-EGF factor 8 antibody O acetyl disialoganglioside synthase antibody OAcGD3S antibody SED1 antibody SPAG10 antibody Sperm associated antigen 10 antibody Sperm surface protein hP47 antibody |
| Molecular Weight(MW) | 43kDa(Observed band size: 43kDa) |
| Cellular Localization | Membrane, Secreted, Cytoplasmic vesicle, secretory vesicle, acrosome membrane. |
WB
Western blot analysis of MFGE8 on different lysates with Rabbit anti-MFGE8 antibody at 1/1000 dilution. Lane 1: A549 cell lysate, Lane 2: A431 cell lysate, Lane 3: HeLa cell lysate, Lane 4: MCF7 cell lysate, Lane 5: SK-Br-3 cell lysate, Lane 6: BT-20 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
