WB
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody at 1/2000 dilution. Lane 1: HCT 116 cell lysate (negative), Lane 2: Raji cell lysate, Lane 3: Ramos cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody at 1/2000 dilution. Lane 1: THP-1 cell lysate, Lane 2: A20 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of A20 cells labeling IRF8 with Rabbit anti-IRF8 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRF8 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HCT 116 (left, negative) and THP-1 (right, positive) cells labeling IRF8. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
IRF8 was immunoprecipitated from 0.2 mg THP-1 cell lysate with Rabbit anti-IRF8 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-IRF8 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input), Lane 2: Rabbit anti-IRF8 antibody IP in THP-1 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-IRF8 antibody in THP-1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds.| Product Name | IRF8 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within human IRF8 aa 81-426. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:2000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IRF8 |
|---|---|
| Gene Synonyms | ICSBP IRF-8 ICSBP1 IMD32A IMD32B H-ICSBP |
| Gene Full Name | interferon regulatory factor 8 |
| Gene Summary | Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008] |
| Alternative Names | H ICSBP antibody H-ICSBP antibody HGNC:5358 antibody HICSBP antibody ICSBP 1 antibody ICSBP antibody ICSBP1 antibody Interferon consensus sequence binding protein 1 antibody Interferon consensus sequence binding protein antibody Interferon consensus sequence-binding protein antibody H ICSBP antibody H-ICSBP antibody HGNC:5358 antibody HICSBP antibody ICSBP 1 antibody ICSBP antibody ICSBP1 antibody Interferon consensus sequence binding protein 1 antibody Interferon consensus sequence binding protein antibody Interferon consensus sequence-binding protein antibody Interferon regulatory factor 8 antibody IRF 8 antibody IRF-8 antibody Irf8 antibody IRF8_HUMAN antibody MYLS antibody |
| Molecular Weight(MW) | 48kDa(Observed band size:50kDa) |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody at 1/2000 dilution. Lane 1: HCT 116 cell lysate (negative), Lane 2: Raji cell lysate, Lane 3: Ramos cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of IRF8 on different lysates with Rabbit anti-IRF8 antibody at 1/2000 dilution. Lane 1: THP-1 cell lysate, Lane 2: A20 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of A20 cells labeling IRF8 with Rabbit anti-IRF8 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRF8 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HCT 116 (left, negative) and THP-1 (right, positive) cells labeling IRF8. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
IRF8 was immunoprecipitated from 0.2 mg THP-1 cell lysate with Rabbit anti-IRF8 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-IRF8 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input), Lane 2: Rabbit anti-IRF8 antibody IP in THP-1 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-IRF8 antibody in THP-1 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds.| Application Notes | WB:1:2000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
