WB
Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody at 1/1000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: SK-OV-3 cell lysate (15 µg/Lane), Lane 3: SW480 cell lysate (15 µg/Lane), Lane 4: 293T cell lysate (15 µg/Lane), Lane 5: MCF7 cell lysate (15 µg/Lane), Lane 6: Mouse kidney tissue lysate (30 µg/Lane), Lane 7: Rat kidney tissue lysate (30 µg/Lane), Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling IGFBP7 with Rabbit anti-IGFBP7 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IGFBP7 antibody at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-IGFBP7 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
ow cytometric analysis of HeLa cells labeling IGFBP7. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | IGFBP7 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within Human IGFBP7 aa 51-250 / 282. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 ICC/IF:1:100 IHC:1:1000 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGFBP7 |
|---|---|
| Gene Synonyms | AGM PSF TAF FSTL2 IBP-7 MAC25 IGFBP-7 RAMSVPS IGFBP-7v IGFBPRP1 |
| Gene Full Name | insulin like growth factor binding protein 7 |
| Gene Summary | This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011] |
| Alternative Names | AGM antibody Angiomodulin antibody FSTL2 antibody IBP-7 antibody IBP7 antibody IBP7_HUMAN antibody IGF binding protein 7 antibody IGF-binding protein 7 antibody IGFBP rP1 antibody IGFBP-7 antibody AGM antibody Angiomodulin antibody FSTL2 antibody IBP-7 antibody IBP7 antibody IBP7_HUMAN antibody IGF binding protein 7 antibody IGF-binding protein 7 antibody IGFBP rP1 antibody IGFBP-7 antibody IGFBP-rP1 antibody IGFBP7 antibody IGFBPRP1 antibody Insulin like growth factor binding protein 7 antibody Insulin-like growth factor-binding protein 7 antibody MAC25 antibody MAC25 protein antibody PGI2 stimulating factor antibody PGI2-stimulating factor antibody Prostacyclin stimulating factor antibody Prostacyclin-stimulating factor antibody PSF antibody RAMSVPS antibody TAF antibody Tumor-derived adhesion factor antibody |
| Molecular Weight(MW) | 29kDa(Observed band size:29kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody at 1/1000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: SK-OV-3 cell lysate (15 µg/Lane), Lane 3: SW480 cell lysate (15 µg/Lane), Lane 4: 293T cell lysate (15 µg/Lane), Lane 5: MCF7 cell lysate (15 µg/Lane), Lane 6: Mouse kidney tissue lysate (30 µg/Lane), Lane 7: Rat kidney tissue lysate (30 µg/Lane), Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling IGFBP7 with Rabbit anti-IGFBP7 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IGFBP7 antibody at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-IGFBP7 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
ow cytometric analysis of HeLa cells labeling IGFBP7. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 ICC/IF:1:100 IHC:1:1000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
