WB
Western blot analysis of various lysates using Histone H3 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μ g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.ICC/IF
Confocal imaging of HeLa cells using Histone H3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3,Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L),Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective:100x.ICC/IF
Confocal imaging of NIH/3T3 cells using Histone H3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.ICC/IF
Immunofluorescence analysis of PC-12 cells using Histone H3 Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of Oryza sativa, using Histone H3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.ChIP
Chromatin immunoprecipitation analysis of extracts of Hela cells, using Histone H3 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Histone H3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:200-1:100 IHC:1:200-1:800 ChIP:5μg antibody for 5μ g-10μ g of Chromatin |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using Histone H3 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μ g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.
ICC/IF
Confocal imaging of HeLa cells using Histone H3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3,Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L),Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective:100x.
ICC/IF
Confocal imaging of NIH/3T3 cells using Histone H3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ICC/IF
Immunofluorescence analysis of PC-12 cells using Histone H3 Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of Oryza sativa, using Histone H3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ChIP
Chromatin immunoprecipitation analysis of extracts of Hela cells, using Histone H3 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:200-1:100 IHC:1:200-1:800 ChIP:5μg antibody for 5μ g-10μ g of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
