WB
Western blot analysis of various lysates using Acetyl-Histone H3-K9 Rabbit mAb at 1:30000 dilution incubated at room temperature for 1.5 hours. HeLa, NIH/3T3 and C6 cells were treated with TSA(1μM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30μg per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time:1s.ICC/IF
Confocal imaging of HeLa cells (treated withTSA) and HeLa cells (untreated) using AcetylHistone H3-K9 Rabbit mAb (dilution1:4800) followed by a further incubation withCy3-conjugated Goat anti-Rabbit IgG (H+L)(dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) (dilution 1:500)(Green). DAPI was used for nuclear staining(Blue). Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells (treatedwith TSA) and NIH/3T3 cells (untreated)using Acetyl-Histone H3-K9 Rabbit mAb(dilution 1:4800) followed by afurther incubation with Cy3-conjugated Goatanti-Rabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin Mouse mAb (dilution 1:400)followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(dilution 1:500) (Green). DAPI wasused for nuclear staining (Blue). Objective:100x.IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingAcetyl-Histone H3-K9 Rabbit mAb at a dilution of 1:2000 (40x lens). Highpressure antigen retrieval performed with0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue using AcetylHistone H3-K9 Rabbit mAb at adilution of 1:2000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat pancreas tissue using AcetylHistone H3-K9 Rabbit mAb at adilution of 1:2000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.DB
Dot-blot analysis of all sorts of peptidesusing Acetyl-Histone H3-K9 antibody at 1:1000 dilution.IP
Immunoprecipitation analysis of 600 μg extracts of NIH/3T3 cells using 5 μg Acetyl-Histone H3-K9antibody. Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K9antibody at a dilution of 1:1000.CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K-562 cells with 1 μg of AcetylHistone H3-K9 Rabbit mAb followed by incubation with Goat Anti-RabbitIgG(H+L). The CUT&Tag resultsdenote the enrichment pattern of AcetylHistone H3-K9 around RPL30 gene.CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K-562 with 1 μg of Acetyl-HistoneH3-K9 Rabbit mAb, followed byincubation with Goat Anti-RabbitIgG(H+L). The CUT&Tag resultsdenote the enrichment pattern of AAcetylHistone H3-K9 Rabbit across chromosome 8(upper panel) and the genomic regionencompassing RPL30, a representative geneenriched in Acetyl-Histone H3-K9 Rabbit(lower panel).| Product Name | Acetyl-Histone H3-K9 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | Acetyl-K9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIHCIPWBCUT&Tag-seq |
| WB:1:30000-1:150000 ICC/IF:1:1000-1:6000 IHC:1:1000-1:4000 ChIP:5μg antibody for 5g-10μg of Chromatin DB:1:500-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells CUT&Tag:10⁵ cells/1μg |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A Acetyl-Histone H3-K9 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using Acetyl-Histone H3-K9 Rabbit mAb at 1:30000 dilution incubated at room temperature for 1.5 hours. HeLa, NIH/3T3 and C6 cells were treated with TSA(1μM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30μg per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time:1s.
ICC/IF
Confocal imaging of HeLa cells (treated withTSA) and HeLa cells (untreated) using AcetylHistone H3-K9 Rabbit mAb (dilution1:4800) followed by a further incubation withCy3-conjugated Goat anti-Rabbit IgG (H+L)(dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) (dilution 1:500)(Green). DAPI was used for nuclear staining(Blue). Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells (treatedwith TSA) and NIH/3T3 cells (untreated)using Acetyl-Histone H3-K9 Rabbit mAb(dilution 1:4800) followed by afurther incubation with Cy3-conjugated Goatanti-Rabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin Mouse mAb (dilution 1:400)followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(dilution 1:500) (Green). DAPI wasused for nuclear staining (Blue). Objective:100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingAcetyl-Histone H3-K9 Rabbit mAb at a dilution of 1:2000 (40x lens). Highpressure antigen retrieval performed with0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue using AcetylHistone H3-K9 Rabbit mAb at adilution of 1:2000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat pancreas tissue using AcetylHistone H3-K9 Rabbit mAb at adilution of 1:2000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
DB
Dot-blot analysis of all sorts of peptidesusing Acetyl-Histone H3-K9 antibody at 1:1000 dilution.
IP
Immunoprecipitation analysis of 600 μg extracts of NIH/3T3 cells using 5 μg Acetyl-Histone H3-K9antibody. Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K9antibody at a dilution of 1:1000.
CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K-562 cells with 1 μg of AcetylHistone H3-K9 Rabbit mAb followed by incubation with Goat Anti-RabbitIgG(H+L). The CUT&Tag resultsdenote the enrichment pattern of AcetylHistone H3-K9 around RPL30 gene.
CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K-562 with 1 μg of Acetyl-HistoneH3-K9 Rabbit mAb, followed byincubation with Goat Anti-RabbitIgG(H+L). The CUT&Tag resultsdenote the enrichment pattern of AAcetylHistone H3-K9 Rabbit across chromosome 8(upper panel) and the genomic regionencompassing RPL30, a representative geneenriched in Acetyl-Histone H3-K9 Rabbit(lower panel).| Application Notes | WB:1:30000-1:150000 ICC/IF:1:1000-1:6000 IHC:1:1000-1:4000 ChIP:5μg antibody for 5g-10μg of Chromatin DB:1:500-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells CUT&Tag:10⁵ cells/1μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
