WB
Western blot analysis of various lysates, using TriMethyl-Histone H3-K27 Rabbit mAb at1:140000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of HeLa cellsusing TriMethyl-Histone H3-K27 Rabbit mAb at dilution of 1:600 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using TriMethylHistone H3-K27 Rabbit mAb atdilution of 1:1000 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitativePCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1μg TriMethylHistone H3-K27 Rabbit mAb, alongwith a Goat Anti-Rabbit IgG(H+L). TheCUT&Tag results indicate the enrichmentpattern of H3K27Me3 in representative geneloci (MYT1), as shown in figure.| Product Name | TriMethyl-Histone H3-K27 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | TriMethyl-K27 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCWBCUT&Tag-seq |
| WB:1:5000-1:10000 ICC/IF:1:500-1:1000 IHC:1:500-1:1000 ChIP:5μg antibody for 5g-10μg of Chromatin CUT&Tag:10⁵ cells/1μg |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3t H3.4 H3/g H3FT H3C16 HIST3H3 TriMethyl-Histone H3-K27 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates, using TriMethyl-Histone H3-K27 Rabbit mAb at1:140000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing TriMethyl-Histone H3-K27 Rabbit mAb at dilution of 1:600 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using TriMethylHistone H3-K27 Rabbit mAb atdilution of 1:1000 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitativePCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag
CUT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1μg TriMethylHistone H3-K27 Rabbit mAb, alongwith a Goat Anti-Rabbit IgG(H+L). TheCUT&Tag results indicate the enrichmentpattern of H3K27Me3 in representative geneloci (MYT1), as shown in figure.| Application Notes | WB:1:5000-1:10000 ICC/IF:1:500-1:1000 IHC:1:500-1:1000 ChIP:5μg antibody for 5g-10μg of Chromatin CUT&Tag:10⁵ cells/1μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
