WB
Western blot analysis of various lysates using Histone H3 Rabbit mAb at 1:160000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.WB
Western blot analysis of lysates from HeLa cells using Histone H3 Rabbit mAb at1:10000-1:1280000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using HistoneH3 Rabbit mAb at a dilution of1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using HistoneH3 Rabbit mAb at a dilution of1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using Histone H3Rabbit mAb at a dilution of 1:5500(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IP
Immunoprecipitation of Histone H3 from 200 µg extracts of MCF7 cells was performed using 2 µg ofHistone H3 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgGsample. IP samples were eluted with 1× Laemmli Buffer. The Input lane represents 10% of the total input.Western blot analysis of immunoprecipitates was conducted using Histone H3 Rabbit mAb at adilution of 1:5000.ChIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa, using 2μg of Histone H3 Rabbit mAb and Rabbit IgG isotype control. The enrichment ofimmunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogramcompares the ratio of the immunoprecipitated DNA to the input at given loci.| Product Name | Histone H3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPIHCIPWB |
| WB:1:80000-1:320000 IHC:1:5000-1:20000 ChIP:2μg antibody for 10μg-15μg of Chromatin IP:0.5μg-4μg antibody for 100μg-300μg extracts of whole cells |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A Histone H3 H3-4 H3/t H3/g H3FT HIST3H3 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, nucleus. |
WB
Western blot analysis of various lysates using Histone H3 Rabbit mAb at 1:160000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.
WB
Western blot analysis of lysates from HeLa cells using Histone H3 Rabbit mAb at1:10000-1:1280000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using HistoneH3 Rabbit mAb at a dilution of1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using HistoneH3 Rabbit mAb at a dilution of1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using Histone H3Rabbit mAb at a dilution of 1:5500(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IP
Immunoprecipitation of Histone H3 from 200 µg extracts of MCF7 cells was performed using 2 µg ofHistone H3 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgGsample. IP samples were eluted with 1× Laemmli Buffer. The Input lane represents 10% of the total input.Western blot analysis of immunoprecipitates was conducted using Histone H3 Rabbit mAb at adilution of 1:5000.
ChIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa, using 2μg of Histone H3 Rabbit mAb and Rabbit IgG isotype control. The enrichment ofimmunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogramcompares the ratio of the immunoprecipitated DNA to the input at given loci.| Application Notes | WB:1:80000-1:320000 IHC:1:5000-1:20000 ChIP:2μg antibody for 10μg-15μg of Chromatin IP:0.5μg-4μg antibody for 100μg-300μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
