WB
Western blot analysis of various lysates, using MonoMethyl-Histone H3-K36 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): H3 proteinExposure time: 60s.WB
Western blot analysis of lysates from Rice using MonoMethyl-Histone H3-K36 Rabbit mAb at1:2000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.DB
Dot-blot analysis of all sorts of peptidesusing MonoMethyl-Histone H3-K36 RabbitmAb at 1:2000 dilution.ChIP
Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using MonoMethyl-Histone H3-K36Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.IP
Immunoprecipitation analysis of 600ug extracts of Hela cells using 5ug MonoMethyl-Histone H3-K36Rabbit mAb. Western blot was performed from the immunoprecipitate using MonoMethylHistone H3-K36 Rabbit mAb at a dilition of 1:5000.| Product Name | MonoMethyl-Histone H3-K36 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | MonoMethyl-K36 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBIPWB |
| WB:1:1000-1:5000 DB:1:1000-1:5000 ChIP:5μg antibody for 5μg-10μg of Chromatin IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A MonoMethyl-Histone H3-K36 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, nucleus. |
WB
Western blot analysis of various lysates, using MonoMethyl-Histone H3-K36 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): H3 proteinExposure time: 60s.
WB
Western blot analysis of lysates from Rice using MonoMethyl-Histone H3-K36 Rabbit mAb at1:2000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
DB
Dot-blot analysis of all sorts of peptidesusing MonoMethyl-Histone H3-K36 RabbitmAb at 1:2000 dilution.
ChIP
Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using MonoMethyl-Histone H3-K36Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
IP
Immunoprecipitation analysis of 600ug extracts of Hela cells using 5ug MonoMethyl-Histone H3-K36Rabbit mAb. Western blot was performed from the immunoprecipitate using MonoMethylHistone H3-K36 Rabbit mAb at a dilition of 1:5000.| Application Notes | WB:1:1000-1:5000 DB:1:1000-1:5000 ChIP:5μg antibody for 5μg-10μg of Chromatin IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
