WB
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.ICC/IF
Immunofluorescence analysis of C6 cellsusing Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.C6 cells weretreated with TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of HeLa cellsusing Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.HeLa cells weretreated with TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.NIH/3T3 cellswere treated with TSA (1 uM) at 37℃ for 18hours. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human gastric cancer usingAcetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using Acetyl-HistoneH3-K18 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using Acetyl-HistoneH3-K18 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 RabbitpAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.ChIP
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 RabbitpAb yantibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.DB
Dot-blot analysis of all sorts of methylationpeptides using Acetyl-Histone H3-K18antibody at 1:1000 dilution.| Product Name | Acetyl-Histone H3-K18 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | Acetyl-K18 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 ChIP:5μg antibody for 5μg-10μg of Chromatin DB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/j H3C1 H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FJ H3C10 H3C11 HIST1H3J Acetyl-Histone H3-K18 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.C6 cells weretreated with TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.HeLa cells weretreated with TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100.NIH/3T3 cellswere treated with TSA (1 uM) at 37℃ for 18hours. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human gastric cancer usingAcetyl-Histone H3-K18 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using Acetyl-HistoneH3-K18 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using Acetyl-HistoneH3-K18 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 RabbitpAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ChIP
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 RabbitpAb yantibody and rabbit IgG.The amount of immunoprecipitated DNA was checked byquantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
DB
Dot-blot analysis of all sorts of methylationpeptides using Acetyl-Histone H3-K18antibody at 1:1000 dilution.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 ChIP:5μg antibody for 5μg-10μg of Chromatin DB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
