WB
Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb at 1:500dilution. HeLa cells and NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of C6 cellsusing Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.C6 cells were treated withTSA (1 uM) at 37℃ for 18 hours. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.NIH/3T3 cells were treatedwith TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U-2 OS cellsusing Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.U2OS cells were treatedwith TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human tonsil using AcetylHistone H3-K23 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat ovary using Acetyl-HistoneH3-K23 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Acetyl-Histone H3-K23 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | Acetyl-K23 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCWB |
| WB:1:100-1:500 ICC/IF:1:50-1:200 IHC:1:50-1:200 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A Acetyl-Histone H3-K23 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb at 1:500dilution. HeLa cells and NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.C6 cells were treated withTSA (1 uM) at 37℃ for 18 hours. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.NIH/3T3 cells were treatedwith TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U-2 OS cellsusing Acetyl-Histone H3-K23 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.U2OS cells were treatedwith TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human tonsil using AcetylHistone H3-K23 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat ovary using Acetyl-HistoneH3-K23 Rabbit pAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:100-1:500 ICC/IF:1:50-1:200 IHC:1:50-1:200 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
