WB
Western blot analysis of various lysates using Apolipoprotein C3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of HepG2 cellsusing Apolipoprotein C3 Rabbit pAb at dilution of 1:200 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using ApolipoproteinC3 Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen using ApolipoproteinC3 Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen usingApolipoprotein C3 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.IF-P
Immunofluorescence analysis of paraffinembedded mouse liver using ApolipoproteinC3 Rabbit pAb at dilution of 1:200(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IF-P
Immunofluorescence analysis of paraffinembedded rat liver using Apolipoprotein C3Rabbit pAb at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Apolipoprotein C3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:100-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOC3 |
|---|---|
| Gene Synonyms | Apo-C3 ApoC-3 APOCIII |
| Gene Full Name | apolipoprotein C3 |
| Gene Summary | This gene encodes a protein component of triglyceride (TG)-rich lipoproteins (TRLs) including very low density lipoproteins (VLDL), high density lipoproteins (HDL) and chylomicrons. The encoded protein plays a role in role in the metabolism of these TRLs through multiple modes. This protein has been shown to promote the secretion of VLDL1, inhibit lipoprotein lipase enzyme activity, and delay catabolism of TRL remnants. Mutations in this gene are associated with low plasma triglyceride levels and reduced risk of ischemic cardiovascular disease, and hyperalphalipoproteinemia, which is characterized by elevated levels of high density lipoprotein (HDL) and HDL cholesterol in human patients. This gene and other related genes comprise an apolipoprotein gene cluster on chromosome 11. [provided by RefSeq, Sep 2017] |
| Molecular Weight(MW) | 11kDa(Observed band size:11kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates using Apolipoprotein C3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of HepG2 cellsusing Apolipoprotein C3 Rabbit pAb at dilution of 1:200 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using ApolipoproteinC3 Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen using ApolipoproteinC3 Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen usingApolipoprotein C3 Rabbit pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffinembedded mouse liver using ApolipoproteinC3 Rabbit pAb at dilution of 1:200(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IF-P
Immunofluorescence analysis of paraffinembedded rat liver using Apolipoprotein C3Rabbit pAb at dilution of 1:200 (40xlens). Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:100-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
