WB
Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3-S28 Rabbit pAb at 1:900 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of lysates from C6 cells, using Phospho-Histone H3-S28 Rabbit pAb at1:900 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S28 Rabbit pAb at 1:900 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes afterserum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 0.5s.WB
Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb at 1:1000dilution. Both HeLa cells and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of H9C2 cellsusing Phospho-Histone H3-S28 Rabbit pAb at dilution of 1:100. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Phospho-Histone H3-S28 RabbitpAb at dilution of 1:100. Blue: DAPIfor nuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cellsusing Phospho-Histone H3-S28 Rabbit pAb at dilution of 1:100. Blue: DAPI fornuclear staining.| Product Name | Phospho-Histone H3-S28 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A Phospho-Histone H3-S28 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3-S28 Rabbit pAb at 1:900 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of lysates from C6 cells, using Phospho-Histone H3-S28 Rabbit pAb at1:900 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S28 Rabbit pAb at 1:900 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes afterserum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 0.5s.
WB
Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb at 1:1000dilution. Both HeLa cells and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of H9C2 cellsusing Phospho-Histone H3-S28 Rabbit pAb at dilution of 1:100. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Phospho-Histone H3-S28 RabbitpAb at dilution of 1:100. Blue: DAPIfor nuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cellsusing Phospho-Histone H3-S28 Rabbit pAb at dilution of 1:100. Blue: DAPI fornuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
