WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H3-T11 Rabbit mAb at 1:5000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes afterserum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-T11 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissueusing Phospho-Histone H3-T11 RabbitmAb at a dilution of 1:300 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using PhosphoHistone H3-T11 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.ChIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells (Calyculin A,100 nM, 30 minutes), using Phospho-Histone H3-T11 Rabbit mAb and rabbit IgG. Theamount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio ofthe immunoprecipitated DNA versus the input.IF-P
Immunofluorescence analysis of paraffinembedded Mouse liver tissue using PhosphoHistone H3-T11 Rabbit mAb at a dilution of 1:300 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.| Product Name | Phospho-Histone H3-T11 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-T11 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPIF-PIHCWB |
| WB:1:2000-1:6000 IHC:1:100-1:500 ChIP:5μg antibody for 5μg-10μg of Chromatin IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H3-T11 Rabbit mAb at 1:5000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes afterserum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-T11 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serumstarvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissueusing Phospho-Histone H3-T11 RabbitmAb at a dilution of 1:300 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using PhosphoHistone H3-T11 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.
ChIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells (Calyculin A,100 nM, 30 minutes), using Phospho-Histone H3-T11 Rabbit mAb and rabbit IgG. Theamount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio ofthe immunoprecipitated DNA versus the input.
IF-P
Immunofluorescence analysis of paraffinembedded Mouse liver tissue using PhosphoHistone H3-T11 Rabbit mAb at a dilution of 1:300 (40x lens). Secondaryantibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.| Application Notes | WB:1:2000-1:6000 IHC:1:100-1:500 ChIP:5μg antibody for 5μg-10μg of Chromatin IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
