WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HeLa cells were treated with Nocodazole (100 ng/mL) at 37℃for 17 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with Nocodazole (100 ng/mL) at37℃ for 17 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30 s.WB
Western blot analysis of lysates from C6 cells using Phospho-Histone H3-S10 Rabbit mAb at1:6000 dilution incubated overnight at 4℃. C6 cells were treated with Nocodazole (100 ng/mL) at 37℃ for17 hours, or treated with Nocodazole (100 ng/mL) at 37℃ for 17 hours and λ-PP (2 U/μL) at 37℃ for 1hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10 s.IHC
Immunohistochemistry analysis of paraffinembedded Human cervix tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat testis tissue, untreated (left)and lambda phosphatase-treated (right),using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IP
Immunoprecipitation of Phospho-Histone H3-S10 from 300 µg extracts of HeLa cells was performed using2 µg of Phospho-Histone H3-S10 Rabbit mAb. Rabbit Control IgG was used toprecipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lanerepresents 10% of the total input. Western blot analysis of immunoprecipitates was conducted usingPhospho-Histone H3-S10 Rabbit mAb at a dilution of 1:10000.DB
Dot-blot analysis of all sorts of peptidesusing Phospho-Histone H3-S10 Rabbit mAb at 1:5000 dilution incubatedovernight at 4℃.| Product Name | Phospho-Histone H3-S10 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S10 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBIHCIPWB |
| WB:1:5000-1:20000 IHC:1:500-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells DB:1:2000-1:5000 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HeLa cells were treated with Nocodazole (100 ng/mL) at 37℃for 17 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20 s.
WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with Nocodazole (100 ng/mL) at37℃ for 17 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30 s.
WB
Western blot analysis of lysates from C6 cells using Phospho-Histone H3-S10 Rabbit mAb at1:6000 dilution incubated overnight at 4℃. C6 cells were treated with Nocodazole (100 ng/mL) at 37℃ for17 hours, or treated with Nocodazole (100 ng/mL) at 37℃ for 17 hours and λ-PP (2 U/μL) at 37℃ for 1hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10 s.
IHC
Immunohistochemistry analysis of paraffinembedded Human cervix tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue, untreated(left) and lambda phosphatase-treated(right), using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat testis tissue, untreated (left)and lambda phosphatase-treated (right),using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:500 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IP
Immunoprecipitation of Phospho-Histone H3-S10 from 300 µg extracts of HeLa cells was performed using2 µg of Phospho-Histone H3-S10 Rabbit mAb. Rabbit Control IgG was used toprecipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lanerepresents 10% of the total input. Western blot analysis of immunoprecipitates was conducted usingPhospho-Histone H3-S10 Rabbit mAb at a dilution of 1:10000.
DB
Dot-blot analysis of all sorts of peptidesusing Phospho-Histone H3-S10 Rabbit mAb at 1:5000 dilution incubatedovernight at 4℃.| Application Notes | WB:1:5000-1:20000 IHC:1:500-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells DB:1:2000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
