WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with PADI4 usingCitrulline-Histone H3-R8 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5hours. 293F cells transfected with PADI4 were treated by CaCl2 (10 mM) and Ionomycin (10 μM) at 37℃for 4 hours after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.DB
Dot-blot analysis of all sorts of peptidesusing Citrulline-Histone H3-R8 Rabbit mAb at 1:5000 dilution.| Product Name | Citrulline-Histone H3-R8 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | Citrulline-R8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1:5000-1:10000 DB:1:1000-1:5000 |
|
| Species Reactivity | HumanOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | extracellular exosome, nucleoplasm, nucleus, Chromosome, Nucleus. |
WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with PADI4 usingCitrulline-Histone H3-R8 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5hours. 293F cells transfected with PADI4 were treated by CaCl2 (10 mM) and Ionomycin (10 μM) at 37℃for 4 hours after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
DB
Dot-blot analysis of all sorts of peptidesusing Citrulline-Histone H3-R8 Rabbit mAb at 1:5000 dilution.| Application Notes | WB:1:5000-1:10000 DB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
