WB
Western blot analysis of Phospho-MLKL (S358) on different lysates with Rabbit anti-Phospho-MLKL (S358) antibody at 1/5000 dilution. Lane 1: HT-29 cell lysate, Lane 2: HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.DB
Dot blot analysis of Phospho-MLKL (S358) on different peptides with Rabbit anti-Phospho-MLKL (S358) antibody at 1/5000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution for 1 hour at room temperature. Lane 1: Unmodified MLKL peptide (negative), Lane 2: Phospho-MLKL (S358) peptide (positive), Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 5 seconds.| Product Name | Phospho-MLKL (S358) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser358 of Human MLKL. |
| Modification | P-S358 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1:5000 DB:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MLKL |
|---|---|
| Gene Synonyms | hMLKL |
| Gene Full Name | mixed lineage kinase domain like pseudokinase |
| Gene Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | 9130019I15Rik antibody FLJ34389 antibody hMLKL antibody Mixed lineage kinase domain like antibody Mixed lineage kinase domain like protein antibody Mixed lineage kinase domain-like protein antibody Mlkl antibody MLKL_HUMAN antibody |
| Molecular Weight(MW) | 54kDa(Observed band size:54kDa) |
| Cellular Localization | Cytoplasm, Cell membrane, Nucleus. |
WB
Western blot analysis of Phospho-MLKL (S358) on different lysates with Rabbit anti-Phospho-MLKL (S358) antibody at 1/5000 dilution. Lane 1: HT-29 cell lysate, Lane 2: HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
DB
Dot blot analysis of Phospho-MLKL (S358) on different peptides with Rabbit anti-Phospho-MLKL (S358) antibody at 1/5000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution for 1 hour at room temperature. Lane 1: Unmodified MLKL peptide (negative), Lane 2: Phospho-MLKL (S358) peptide (positive), Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 5 seconds.| Application Notes | WB:1:5000 DB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
