WB
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody at 1/2000 dilution. Lane 1: Mouse white adipose tissue lysate, Lane 2: Rat white adipose tissue lysate, Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 40 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser660 of Human HSL. |
| Modification | P-S660 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LIPE |
|---|---|
| Gene Synonyms | HSL LHS REH AOMS4 FPLD6 |
| Gene Full Name | lipase E, hormone sensitive type |
| Gene Summary | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
| Alternative Names | Hormone sensitive lipase antibody Hormone sensitive lipase testicular isoform antibody Hormone-sensitive lipase antibody HSL antibody LHS antibody Lipase hormone sensitive antibody LIPE antibody LIPS_HUMAN antibody |
| Molecular Weight(MW) | 117kDa(Observed band size:84kDa) |
| Cellular Localization | Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet. |
WB
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody at 1/2000 dilution. Lane 1: Mouse white adipose tissue lysate, Lane 2: Rat white adipose tissue lysate, Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 40 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
