WB
Western blot analysis of TCF7 on different lysates with Rabbit anti-TCF7 antibody at 1/5000 dilution. Lane 1: MOLT-4 cell lysate (20 µg/Lane), Lane 3: Jurkat cell lysate (20 µg/Lane), Lane 2: COLO205 cell lysate (20 µg/Lane), Lane 4: LNCaP cell lysate (negative) (20 µg/Lane), Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
TCF7 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-TCF7 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TCF7 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2:Rabbit anti-TCF7 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TCF7 antibodyin Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 25 seconds.| Product Name | TCF7 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within human TCF7 aa 1-300. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TCF7 |
|---|---|
| Gene Synonyms | TCF-1 |
| Gene Full Name | transcription factor 7 |
| Gene Summary | This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016] |
| Alternative Names | FLJ36364 antibody MGC47735 antibody OTTHUMP00000159391 antibody T cell factor 1 antibody T cell specific transcription factor 1 antibody T-cell factor 1 antibody T-cell-specific transcription factor 1 antibody TCF-1 antibody TCF-7 antibody TCF1 antibody FLJ36364 antibody MGC47735 antibody OTTHUMP00000159391 antibody T cell factor 1 antibody T cell specific transcription factor 1 antibody T-cell factor 1 antibody T-cell-specific transcription factor 1 antibody TCF-1 antibody TCF-7 antibody TCF1 antibody Tcf7 antibody TCF7 transcription factor 7 antibody TCF7_HUMAN antibody Transcription factor 7 (T cell specific HMG box) antibody Transcription factor 7 antibody Transcription factor-7 T-cell specific antibody Transcription factor-7 T-cell specific 1 antibody |
| Molecular Weight(MW) | 42kDa(Observed band size:45-50kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of TCF7 on different lysates with Rabbit anti-TCF7 antibody at 1/5000 dilution. Lane 1: MOLT-4 cell lysate (20 µg/Lane), Lane 3: Jurkat cell lysate (20 µg/Lane), Lane 2: COLO205 cell lysate (20 µg/Lane), Lane 4: LNCaP cell lysate (negative) (20 µg/Lane), Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat lymph node tissue with Rabbit anti-TCF7 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
TCF7 was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-TCF7 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TCF7 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2:Rabbit anti-TCF7 antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TCF7 antibodyin Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 25 seconds.| Application Notes | WB:1:5000 IHC:1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
