WB
Western blot analysis of Hsp90 beta on different lysates with Rabbit anti-Hsp90 beta antibody at 1/10000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: K-562 cell lysate (20 µg/Lane), Lane 5: RAW264.7 cell lysate (20 µg/Lane), Lane 6: PC-12 cell lysate (20 µg/Lane), Lane 7: Mouse heart tissue lysate (40 µg/Lane), Lane 8: Rat heart tissue lysate (40 µg/Lane), Lane 9: Human liver tissue lysate (40 µg/Lane), Lane 10: Mouse liver tissue lysate (40 µg/Lane), Lane 11: Rat liver tissue lysate (40 µg/Lane), Lane 12: Human kidney tissue lysate (40 µg/Lane), Lane 13: Mouse kidney tissue lysate (40 µg/Lane), Lane 14: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Hsp90 beta on different lysates with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-Hsp90 beta KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Hsp90 beta with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Hsp90 beta with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hsp90 beta antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp90 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HeLa cells labeling Hsp90 beta. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Hsp90 beta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within Human Hsp90 beta aa 485-724 / 724. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:20000 IHC:1:400 ICC/IF:1:1000 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSP90AB1 |
|---|---|
| Gene Synonyms | HSP84 HSPC2 HSPCB D6S182 HSP90B |
| Gene Full Name | heat shock protein 90 alpha family class B member 1 |
| Gene Summary | This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012] |
| Alternative Names | 90 kda heat shock protein beta HSP90 beta antibody D6S182 antibody FLJ26984 antibody Heat shock 84 kDa antibody Heat shock 90kD protein 1 beta antibody Heat shock 90kDa protein 1 beta antibody Heat shock protein 90 alpha family class B member 1 antibody Heat shock protein 90 kDa antibody Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody Heat shock protein 90kDa alpha family class B member 1 antibody 90 kda heat shock protein beta HSP90 beta antibody D6S182 antibody FLJ26984 antibody Heat shock 84 kDa antibody Heat shock 90kD protein 1 beta antibody Heat shock 90kDa protein 1 beta antibody Heat shock protein 90 alpha family class B member 1 antibody Heat shock protein 90 kDa antibody Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody Heat shock protein 90kDa alpha family class B member 1 antibody Heat shock protein beta antibody Heat shock protein HSP 90 beta antibody Heat shock protein HSP 90-beta antibody HS90B_HUMAN antibody HSP 84 antibody HSP 90 antibody HSP 90 b antibody HSP 90b antibody HSP84 antibody HSP90 BETA antibody hsp90ab1 antibody HSP90B antibody HSPC2 antibody HSPCB antibody |
| Molecular Weight(MW) | 83kDa(Observed band size:90kDa) |
| Cellular Localization | Cytoplasm, Melanosome,Nucleus,Secreted,Cell membrane,Dynein axonemal particle . |
WB
Western blot analysis of Hsp90 beta on different lysates with Rabbit anti-Hsp90 beta antibody at 1/10000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: K-562 cell lysate (20 µg/Lane), Lane 5: RAW264.7 cell lysate (20 µg/Lane), Lane 6: PC-12 cell lysate (20 µg/Lane), Lane 7: Mouse heart tissue lysate (40 µg/Lane), Lane 8: Rat heart tissue lysate (40 µg/Lane), Lane 9: Human liver tissue lysate (40 µg/Lane), Lane 10: Mouse liver tissue lysate (40 µg/Lane), Lane 11: Rat liver tissue lysate (40 µg/Lane), Lane 12: Human kidney tissue lysate (40 µg/Lane), Lane 13: Mouse kidney tissue lysate (40 µg/Lane), Lane 14: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Hsp90 beta on different lysates with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-Hsp90 beta KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Hsp90 beta with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Hsp90 beta with Rabbit anti-Hsp90 beta antibody at 1/1000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hsp90 beta antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp90 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Hsp90 beta antibody at 1/20000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HeLa cells labeling Hsp90 beta. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000-1:20000 IHC:1:400 ICC/IF:1:1000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
