WB
Western blot analysis of SA2 on different lysates with Rabbit anti-SA2 antibody at 1/2000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: A431 cell lysate (20 µg/Lane), Lane 3: PANC-1 cell lysate (20 µg/Lane), Lane 4: HepG2 cell lysate (20 µg/Lane), Lane 5: Jurkat cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: K-562 cell lysate (20 µg/Lane), Lane 8: HCT 116 cell lysate (20 µg/Lane), Lane 9: COS-1 cell lysate (20 µg/Lane), Lane 10: NIH/3T3 cell lysate (20 µg/Lane), Lane 11: RAW264.7 cell lysate (20 µg/Lane), Lane 12: C6 cell lysate (20 µg/Lane), Lane 13: Mouse spleen tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling SA2 with Rabbit anti-SA2 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SA2 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SA2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within Human SA2 aa 982-1,231 / 1,231. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 IHC:1:500-1:2000 ICC/IF:1:100 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STAG2 |
|---|---|
| Gene Synonyms | SA2 MKMS SA-2 HPE13 SCC3B NEDXCF bA517O1.1 |
| Gene Full Name | STAG2 cohesin complex component |
| Gene Summary | The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| Alternative Names | bA517O1.1 antibody Cohesin Subunit SA 2 antibody Cohesin subunit SA-2 antibody DKFZp686P168 antibody DKFZp781H1753 antibody FLJ25871 antibody SA 2 antibody SA-2 antibody SA2 antibody SCC3 homolog 2 antibody bA517O1.1 antibody Cohesin Subunit SA 2 antibody Cohesin subunit SA-2 antibody DKFZp686P168 antibody DKFZp781H1753 antibody FLJ25871 antibody SA 2 antibody SA-2 antibody SA2 antibody SCC3 homolog 2 antibody SCC3B antibody STAG 2 antibody stag2 antibody STAG2_HUMAN antibody Stromal antigen 2 antibody |
| Molecular Weight(MW) | 141kDa(Observed band size:141kDa) |
| Cellular Localization | Nucleus. Chromosome. |
WB
Western blot analysis of SA2 on different lysates with Rabbit anti-SA2 antibody at 1/2000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: A431 cell lysate (20 µg/Lane), Lane 3: PANC-1 cell lysate (20 µg/Lane), Lane 4: HepG2 cell lysate (20 µg/Lane), Lane 5: Jurkat cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: K-562 cell lysate (20 µg/Lane), Lane 8: HCT 116 cell lysate (20 µg/Lane), Lane 9: COS-1 cell lysate (20 µg/Lane), Lane 10: NIH/3T3 cell lysate (20 µg/Lane), Lane 11: RAW264.7 cell lysate (20 µg/Lane), Lane 12: C6 cell lysate (20 µg/Lane), Lane 13: Mouse spleen tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling SA2 with Rabbit anti-SA2 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SA2 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-SA2 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:500-1:2000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
