WB
Western blot analysis of lysates from Mouse heart, using DR5/TRAILR2/TNFRSF10B Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.WB
Western blot analysis of various lysates using DR5/TRAILR2/TNFRSF10B Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.ICC/IF
Confocal imaging of NIH-3T3 cells usingDR5/TRAILR2/TNFRSF10B Rabbit mAb(dilution 1:100)(Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) (Green). DAPI wasused for nuclear staining (blue). Objective:100x.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingDR5/TRAILR2/TNFRSF10B Rabbit mAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Product Name | DR5/TRAILR2/TNFRSF10B Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:400 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF10B |
|---|---|
| Gene Synonyms | DR5 CD262 KILLER TRICK2 TRICKB ZTNFR9 TRAILR2 TRICK2A TRICK2B TRAIL-R2 KILLER/DR5 |
| Gene Full Name | TNF receptor superfamily member 10b |
| Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009] |
| Alternative Names | DR5 CD262 KILLER TRICK2 TRICKB ZTNFR9 TRAILR2 TRICK2A TRICK2B TRAIL-R2 KILLER/DR5 DR5/TRAILR2/TNFRSF10B |
| Molecular Weight(MW) | 48kDa(Observed band size:48kDa) |
| Cellular Localization | Membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from Mouse heart, using DR5/TRAILR2/TNFRSF10B Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
WB
Western blot analysis of various lysates using DR5/TRAILR2/TNFRSF10B Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.
ICC/IF
Confocal imaging of NIH-3T3 cells usingDR5/TRAILR2/TNFRSF10B Rabbit mAb(dilution 1:100)(Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) (Green). DAPI wasused for nuclear staining (blue). Objective:100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingDR5/TRAILR2/TNFRSF10B Rabbit mAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:400 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
