WB
Western blot analysis of various lysates using Homer1 Rabbit mAb at 1:1000 dilution incubatedovernight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using Homer1Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using Homer1Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman brain tissue using Homer1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Ratbrain tissue using Homer1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | Homer1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:2000 IF-P:1:50-1:200 IHC:1:200-1:800 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOMER1 |
|---|---|
| Gene Synonyms | HOMER SYN47 Ves-1 HOMER1A HOMER1B HOMER1C |
| Gene Full Name | homer scaffold protein 1 |
| Gene Summary | This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008] |
| Alternative Names | HOMER SYN47 Ves-1 HOMER1A HOMER1B HOMER1C |
| Molecular Weight(MW) | 40kDa(Observed band size:46kDa) |
| Cellular Localization | Cell junction, Cytoplasm, postsynaptic cell membrane, postsynaptic density, synapse. |
WB
Western blot analysis of various lysates using Homer1 Rabbit mAb at 1:1000 dilution incubatedovernight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using Homer1Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using Homer1Rabbit mAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman brain tissue using Homer1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Ratbrain tissue using Homer1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:1000-1:2000 IF-P:1:50-1:200 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
