WB
Western blot analysis of various lysates using FABPI Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffinembedded Rat intestine using FABPI RabbitmAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHCstaining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin using FABPI RabbitmAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedmouse small intestine using FABPI RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500)(Red).DAPIwas used for nuclear staining (Blue).Objective: 40x.Perform high pressure antigenretrieval with 0.01M citrate buffer (pH 6.0)prior to IF staining.| Product Name | FABPI Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:2000 IF-P:1:100-1:1000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FABP2 |
|---|---|
| Gene Synonyms | FABPI I-FABP |
| Gene Full Name | fatty acid binding protein 2 |
| Gene Summary | The protein encoded by this gene is an intracellular fatty acid-binding protein that participates in the uptake, intracellular metabolism, and transport of long-chain fatty acids. The encoded protein is also involved in the modulation of cell growth and proliferation. This protein binds saturated long-chain fatty acids with high affinity, and may act as a lipid sensor to maintain energy homeostasis. [provided by RefSeq, Aug 2017] |
| Alternative Names | FABPI I-FABP |
| Molecular Weight(MW) | 15kDa(Observed band size:14kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of various lysates using FABPI Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat intestine using FABPI RabbitmAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHCstaining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin using FABPI RabbitmAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedmouse small intestine using FABPI RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500)(Red).DAPIwas used for nuclear staining (Blue).Objective: 40x.Perform high pressure antigenretrieval with 0.01M citrate buffer (pH 6.0)prior to IF staining.| Application Notes | WB:1:1000-1:2000 IF-P:1:100-1:1000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
