WB
Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KDValidated] TFEB Rabbit pAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of various lysates, using [KD Validated] TFEB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cellsusing [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat testis tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining| Product Name | [KD Validated] TFEB Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFEB |
|---|---|
| Gene Synonyms | TCFEB BHLHE35 ALPHATFEB |
| Gene Full Name | transcription factor EB |
| Gene Summary | Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including antibacterial innate immune response; cellular response to amino acid starvation; and positive regulation of metabolic process. Located in cytosol and lysosomal membrane. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TCFEB BHLHE35 ALPHATFEB TFEB |
| Molecular Weight(MW) | 53kDa(Observed band size:65-70kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KDValidated] TFEB Rabbit pAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of various lysates, using [KD Validated] TFEB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cellsusing [KD Validated] TFEB Rabbit pAb at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat testis tissue using [KDValidated] TFEB Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining| Application Notes | WB:1:500-1:5000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
