WB
Western blot analysis of various lysates using EEF1A2 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using EEF1A2 Rabbit pAb at dilution of 1:50 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing EEF1A2 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.| Product Name | EEF1A2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EEF1A2 |
|---|---|
| Gene Synonyms | HS1 STN EF1A STNL DEE33 MRD38 EEF1AL EIEE33 EF-1-alpha-2 |
| Gene Full Name | eukaryotic translation elongation factor 1 alpha 2 |
| Gene Summary | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014] |
| Alternative Names | HS1 STN EF1A STNL DEE33 MRD38 EEF1AL EIEE33 EF-1-alpha-2 EEF1A2 |
| Molecular Weight(MW) | 50kDa(Observed band size:50kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using EEF1A2 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using EEF1A2 Rabbit pAb at dilution of 1:50 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing EEF1A2 Rabbit pAb at dilutionof 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using EEF1A2Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
