WB
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866/S870 Rabbit mAb at 1:4000 dilution. NIH/3T3 cells were treated with TNF-α (20 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingPhospho-NF-κB2-S866/S870 Rabbit mAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Product Name | Phospho-NF-κB2-S866/S870 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S870 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKB2 |
|---|---|
| Gene Synonyms | p52 p100 H2TF1 LYT10 CVID10 LYT-10 NF-kB2 p49/p100 |
| Gene Full Name | nuclear factor kappa B subunit 2 |
| Gene Summary | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| Alternative Names | p52 p100 H2TF1 LYT10 CVID10 LYT-10 NF-kB2 p49/p100 Phospho-NF-κB2-S866/S870 |
| Molecular Weight(MW) | 97kDa(Observed band size:110kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866/S870 Rabbit mAb at 1:4000 dilution. NIH/3T3 cells were treated with TNF-α (20 ng/ml) at 37℃ for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingPhospho-NF-κB2-S866/S870 Rabbit mAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
