WB
Western blot analysis of lysates from wild type (WT) and FOXK1 knockdown (KD) HeLa cells using [KDValidated] FOXK1 mAb at 1:6000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.ICC/IF
Confocal imaging of HeLa cells using [KDValidated] FOXK1 mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells using [KDValidated] FOXK1 mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using [KDValidated] FOXK1 mAb at a dilutionof 1:6000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IP
Immunoprecipitation of from 300 µg extracts of Raji cells was performed using 2 µg of [KD Validated]FOXK1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample.IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Westernblot analysis of immunoprecipitates was conducted using [KD Validated] FOXK1 Rabbit mAb at adilution of 1:3000.ChIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from 293F cells,using 3 μg of [KD Validated] FOXK1 mAb and Rabbit IgG isotype control. Theenrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. Thehistogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Product Name | [KD Validated] FOXK1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCIPWB |
| WB:1:6000-1:24000 ICC/IF:1:200-1:400 IHC:1:2000-1:8000 ChIP:3μg antibody for 10μg-15μg of Chromatin IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXK1 |
|---|---|
| Gene Synonyms | FOXK1L |
| Gene Full Name | forkhead box K1 |
| Gene Summary | Enables 14-3-3 protein binding activity; DNA-binding transcription repressor activity, RNA polymerase II-specific; and transcription cis-regulatory region binding activity. Involved in several processes, including intracellular glucose homeostasis; negative regulation of autophagy; and regulation of DNA-templated transcription. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | FOXK1L |
| Molecular Weight(MW) | 75kDa(Observed band size:97kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of lysates from wild type (WT) and FOXK1 knockdown (KD) HeLa cells using [KDValidated] FOXK1 mAb at 1:6000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
ICC/IF
Confocal imaging of HeLa cells using [KDValidated] FOXK1 mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells using [KDValidated] FOXK1 mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue using [KDValidated] FOXK1 mAb at a dilutionof 1:6000 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IP
Immunoprecipitation of from 300 µg extracts of Raji cells was performed using 2 µg of [KD Validated]FOXK1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample.IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Westernblot analysis of immunoprecipitates was conducted using [KD Validated] FOXK1 Rabbit mAb at adilution of 1:3000.
ChIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from 293F cells,using 3 μg of [KD Validated] FOXK1 mAb and Rabbit IgG isotype control. Theenrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. Thehistogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Application Notes | WB:1:6000-1:24000 ICC/IF:1:200-1:400 IHC:1:2000-1:8000 ChIP:3μg antibody for 10μg-15μg of Chromatin IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
