WB
Western blot analysis of various lysates using AHR Rabbit PolymAb® at 1:10000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): JurkatExposure time: 20s.ICC/IF
Immunofluorescence analysis of MCF7 cellsusing AHR Rabbit PolymAb® ata dilution of 1:100 (40x lens). Secondaryantibody: Cy3 Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human placenta tissue using AHR Rabbit PolymAb® at a dilutionof 1:500 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.| Product Name | AHR Rabbit PolymAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AHR |
|---|---|
| Gene Synonyms | FVH3 RP85 bHLHe76 |
| Gene Full Name | aryl hydrocarbon receptor |
| Gene Summary | The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015] |
| Alternative Names | RP85 bHLHe76 |
| Molecular Weight(MW) | 96kDa(Observed band size:100kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using AHR Rabbit PolymAb® at 1:10000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): JurkatExposure time: 20s.
ICC/IF
Immunofluorescence analysis of MCF7 cellsusing AHR Rabbit PolymAb® ata dilution of 1:100 (40x lens). Secondaryantibody: Cy3 Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human placenta tissue using AHR Rabbit PolymAb® at a dilutionof 1:500 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.| Application Notes | WB:1:2000-1:10000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
