WB
Western blot analysis of lysates from HeLa cells using PLCB1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using PLCB1 Rabbit pAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Product Name | PLCB1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCB1 |
|---|---|
| Gene Synonyms | DEE12 PLC-I EIEE12 PI-PLC PLC154 PLCB1A PLCB1B PLC-154 PLC-beta-1 |
| Gene Full Name | phospholipase C beta 1 |
| Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | DEE12 PLC-I EIEE12 PI-PLC PLC154 PLCB1A PLCB1B PLC-154 PLC-beta-1 PLCB1 |
| Molecular Weight(MW) | 139kDa(Observed band size:150kDa) |
| Cellular Localization | Cytoplasm, Nucleus membrane. |
WB
Western blot analysis of lysates from HeLa cells using PLCB1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using PLCB1 Rabbit pAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
