WB
Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody at 1/5000 dilution. Lane 1: PC-3 cell lysate, Lane 2: K-562 cell lysate (negative), Lane 3: Mouse brain tissue lysate, Lane 4: Mouse skeletal muscle tissue lysate (negative), Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds.4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | APP/beta Amyloid Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Amyloid Precursor Protein aa 671-770 / 770. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:1000-1:2000 IF-F:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APP |
|---|---|
| Gene Synonyms | AAA AD1 PN2 ABPP APPI CVAP ABETA PN-II preA4 CTFgamma alpha-sAPP |
| Gene Full Name | amyloid beta precursor protein |
| Gene Summary | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014] |
| Alternative Names | A4 amyloid protein antibody A4_HUMAN antibody AAA antibody ABETA antibody ABPP antibody AD1 antibody AICD-50 antibody AICD-57 antibody AICD-59 antibody AID(50) antibody A4 amyloid protein antibody A4_HUMAN antibody AAA antibody ABETA antibody ABPP antibody AD1 antibody AICD-50 antibody AICD-57 antibody AICD-59 antibody AID(50) antibody AID(57) antibody AID(59) antibody Alzheimer disease amyloid protein antibody Amyloid beta (A4) precursor protein antibody Amyloid beta A4 protein antibody Amyloid beta protein antibody Amyloid intracellular domain 50 antibody Amyloid intracellular domain 57 antibody Amyloid intracellular domain 59 antibody Amyloid precursor protein antibody APP antibody APPI antibody Beta-amyloid precursor protein antibody C31 antibody Cerebral vascular amyloid peptide antibody CTFgamma antibody CVAP antibody Gamma-CTF(50) antibody Gamma-CTF(57) antibody Gamma-CTF(59) antibody peptidase nexin II antibody peptidase nexin-II antibody PN 2 antibody PN II antibody PN-II antibody PN2 antibody PreA4 antibody Protease nexin II antibody Protease nexin-II antibody S-APP-alpha antibody S-APP-beta antibody sAPP antibody |
| Molecular Weight(MW) | 87kDa(Observed band size:100kDa) |
| Cellular Localization | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
WB
Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody at 1/5000 dilution. Lane 1: PC-3 cell lysate, Lane 2: K-562 cell lysate (negative), Lane 3: Mouse brain tissue lysate, Lane 4: Mouse skeletal muscle tissue lysate (negative), Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds.4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-APP/beta Amyloid antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:1000-1:2000 IF-F:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
