WB
Western blot analysis of lysates from Raji cells using ADORA2A Rabbit pAb at 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of C6 cellsusing ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.ICC/IF
Immunofluorescence analysis of HeLa cellsusing ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.| Product Name | ADORA2A Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADORA2A |
|---|---|
| Gene Synonyms | A2aR RDC8 ADORA2 |
| Gene Full Name | adenosine A2a receptor |
| Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013] |
| Alternative Names | A2aR RDC8 ADORA2 ADORA2A |
| Molecular Weight(MW) | 45kDa(Observed band size:45-60kDa) |
| Cellular Localization | Cell membrane, Multi-pass membrane protein. |
WB
Western blot analysis of lysates from Raji cells using ADORA2A Rabbit pAb at 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ADORA2A Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH7.3. |
