WB
Western blot analysis of lysates from wild type (WT) and c-Myc knockout (KO) HCT 116 cells, using [KOValidated] c-Myc Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of various lysates using [KO Validated] c-Myc Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ChIP
Chromatin immunoprecipitation analysis of extracts of K562 cells, using c-Myc antibody andrabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram wasconstructed by the ratios of the immunoprecipitated DNA to the input.CUT&Tag
UT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1μg c-Myc Rabbit mAb, along with a Goat Anti-RabbitIgG(H+L). The CUT&Tag results indicate theenrichment pattern of ESE1 in representativegene loci (NPM1), as shown in figure.| Product Name | [KO Validated] c-Myc Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Nucleus, nucleolus, nucleoplasm. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPWBCUT&Tag-seq |
| WB:1:1000-1:2000 ChIP:5μg antibody for 10μg-15μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYC |
|---|---|
| Gene Synonyms | MRTL MYCC c-Myc bHLHe39 |
| Gene Full Name | MYC proto-oncogene, bHLH transcription factor |
| Gene Summary | This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017] |
| Alternative Names | MRTL MYCC c-Myc bHLHe39 yc |
| Molecular Weight(MW) | 51kDa(Observed band size:50-60kDa) |
| Cellular Localization | Nucleus, nucleolus, nucleoplasm. |
WB
Western blot analysis of lysates from wild type (WT) and c-Myc knockout (KO) HCT 116 cells, using [KOValidated] c-Myc Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of various lysates using [KO Validated] c-Myc Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ChIP
Chromatin immunoprecipitation analysis of extracts of K562 cells, using c-Myc antibody andrabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram wasconstructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag
UT&Tag was performed using the CUT&TagAssay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1μg c-Myc Rabbit mAb, along with a Goat Anti-RabbitIgG(H+L). The CUT&Tag results indicate theenrichment pattern of ESE1 in representativegene loci (NPM1), as shown in figure.| Application Notes | WB:1:1000-1:2000 ChIP:5μg antibody for 10μg-15μg of Chromatin CUT&Tag:10⁵ cells /1 μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
