WB
Western blot analysis of lysates from K-562 cells, using KLHDC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 150s.IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer tissue usingKLHDC2 Rabbit pAb at a dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver tissue using KLHDC2Rabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Product Name | KLHDC2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHDC2 |
|---|---|
| Gene Synonyms | LCP HCLP1 HCLP-1 |
| Gene Full Name | kelch domain containing 2 |
| Gene Summary | Enables ubiquitin-like ligase-substrate adaptor activity. Involved in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway. Located in nuclear body and nuclear membrane. Is active in Cul2-RING ubiquitin ligase complex and nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | LCP HCLP1 HCLP-1 KLHDC2 |
| Molecular Weight(MW) | 46kDa(Observed band size:46kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of lysates from K-562 cells, using KLHDC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 150s.
IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer tissue usingKLHDC2 Rabbit pAb at a dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse liver tissue using KLHDC2Rabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
