IHC
Immunohistochemistry analysis of paraffinembedded Human anaplastic large celllymphoma tissue using MUM1 Rabbit mAb at dilution of 1:400 (40x lens). Highpressure antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman tonsil tissue using MUM1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IF-P
Confocal imaging of paraffin-embeddedHuman appendix tissue using MUM1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | MUM1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHC |
| IF-P:1:200-1:800 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF4 |
|---|---|
| Gene Synonyms | MUM1 LSIRF SHEP8 IMD131 NF-EM5 |
| Gene Full Name | interferon regulatory factor 4 |
| Gene Summary | The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010] |
| Alternative Names | MUM1 LSIRF SHEP8 NF-EM5 IRF4 |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Nucleus. |
IHC
Immunohistochemistry analysis of paraffinembedded Human anaplastic large celllymphoma tissue using MUM1 Rabbit mAb at dilution of 1:400 (40x lens). Highpressure antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman tonsil tissue using MUM1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embeddedHuman appendix tissue using MUM1 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | IF-P:1:200-1:800 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
