WB
Western blot analysis of various lysates using IDH2 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of C6 cellsusing IDH2 Rabbit mAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of NIH-3T3cells using IDH2 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingIDH2 Rabbit mAb at a dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.| Product Name | IDH2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:200-1:800 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IDH2 |
|---|---|
| Gene Synonyms | IDH IDP IDHM IDPM ICD-M IDH-2 D2HGA2 mNADP-IDH |
| Gene Full Name | isocitrate dehydrogenase (NADP(+)) 2 |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| Alternative Names | IDH IDP IDHM IDPM ICD-M IDH-2 D2HGA2 mNADP-IDH IDH2 |
| Molecular Weight(MW) | 51kDa(Observed band size:43kDa) |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of various lysates using IDH2 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing IDH2 Rabbit mAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of NIH-3T3cells using IDH2 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingIDH2 Rabbit mAb at a dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.| Application Notes | WB:1:1000-1:6000 IHC:1:200-1:800 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
