WB
Western blot analysis of various lysates using P2Y12 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.| Product Name | P2Y12 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:4000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | P2ry12 |
|---|---|
| Gene Synonyms | P2Y12 2900079B22Rik 4921504D23Rik |
| Gene Full Name | purinergic receptor P2Y, G-protein coupled 12 |
| Gene Summary | Enables G protein-coupled ADP receptor activity and G protein-coupled adenosine receptor activity. Involved in several processes, including cellular response to ATP; regulation of microglial cell migration; and substrate-dependent cell migration, cell extension. Acts upstream of or within adenylate cyclase-modulating G protein-coupled receptor signaling pathway; platelet activation; and positive regulation of monoatomic ion transport. Located in cell body membrane and cell projection membrane. Is expressed in central nervous system and cerebral cortex. Used to study platelet-type bleeding disorder 8. Human ortholog(s) of this gene implicated in asthma; cerebrovascular disease; peripheral artery disease; platelet-type bleeding disorder 8; and type 2 diabetes mellitus. Orthologous to human P2RY12 (purinergic receptor P2Y12). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | P2Y12 2900079B22Rik 4921504D23Rik |
| Molecular Weight(MW) | 39kDa(Observed band size:55kDa) |
| Cellular Localization | basal plasma membrane, caveola, cell body membrane, external side of plasma membrane, mitochondrion, plasma membrane. |
WB
Western blot analysis of various lysates using P2Y12 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.| Application Notes | WB:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
