WB
Western blot analysis of various lysates using p70 S6 Kinase 1 Rabbit mAb at 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.ICC/IF
Confocal imaging of 293T cells using p70 S6Kinase 1 Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells using p70S6 Kinase 1 Rabbit mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of C6 cells using p70 S6Kinase 1 Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). The cells were counterstainedwith α-Tubulin Mouse mAb (dilution1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L)Ab (dilution 1:500) (Green). DAPI wasused for nuclear staining (Blue). Objective:100x.| Product Name | p70 S6 Kinase 1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:4000 ICC/IF:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB1 |
|---|---|
| Gene Synonyms | S6K PS6K S6K1 STK14A p70-S6K p70 S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha |
| Gene Full Name | ribosomal protein S6 kinase B1 |
| Gene Summary | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| Alternative Names | S6K PS6K S6K1 STK14A p70-S6K p70 S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha p70 S6 Kinase 1 |
| Molecular Weight(MW) | 59kDa(Observed band size:68/75kDa) |
| Cellular Localization | Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome. |
WB
Western blot analysis of various lysates using p70 S6 Kinase 1 Rabbit mAb at 1:1000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
ICC/IF
Confocal imaging of 293T cells using p70 S6Kinase 1 Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells using p70S6 Kinase 1 Rabbit mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of C6 cells using p70 S6Kinase 1 Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). The cells were counterstainedwith α-Tubulin Mouse mAb (dilution1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L)Ab (dilution 1:500) (Green). DAPI wasused for nuclear staining (Blue). Objective:100x.| Application Notes | WB:1:1000-1:4000 ICC/IF:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
