WB
Western blot analysis of various lysates using Cullin 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HeLa cellsusing Cullin 1 Rabbit pAb. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon using Cullin 1Rabbit pAb at dilution of 1:50 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | Cullin 1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:10-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUL1 |
|---|---|
| Gene Full Name | cullin 1 |
| Gene Summary | Enables ubiquitin ligase complex scaffold activity and ubiquitin protein ligase binding activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of canonical NF-kappaB signal transduction; and protein K48-linked ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Cullin 1 CUL1 cullin-1 MGC149834 MGC149835 |
| Molecular Weight(MW) | 90kDa(Observed band size:80kDa) |
| Cellular Localization | cytosol, nucleoplasm, plasma membrane. |
WB
Western blot analysis of various lysates using Cullin 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing Cullin 1 Rabbit pAb. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon using Cullin 1Rabbit pAb at dilution of 1:50 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:10-1:100 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
