WB
Western blot analysis of lysates from HL-60 cells using Myeloperoxidase (MPO) Rabbit mAb at1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC):HeLaExposure time: 30s.ICC/IF
Confocal imaging of HL-60 cells usingMyeloperoxidase (MPO) Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing Myeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue usingMyeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue usingMyeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IF-P
Confocal imaging of paraffin-embedded Ratspleen tissue using Myeloperoxidase (MPO)Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embeddedMouse spleen tissue using Myeloperoxidase(MPO) Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embeddedHuman colon tissue using Myeloperoxidase(MPO) Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Product Name | Myeloperoxidase (MPO) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:3000-1:10000 ICC/IF:1:50-1:200 IHC:1:5000-1:20000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPO |
|---|---|
| Gene Full Name | myeloperoxidase |
| Gene Summary | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Alternative Names | MPO myeloperoxidase Myeloperoxidase (MPO) |
| Molecular Weight(MW) | 84kDa(Observed band size:60/80-90kDa) |
| Cellular Localization | Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome. |
WB
Western blot analysis of lysates from HL-60 cells using Myeloperoxidase (MPO) Rabbit mAb at1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC):HeLaExposure time: 30s.
ICC/IF
Confocal imaging of HL-60 cells usingMyeloperoxidase (MPO) Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Objective: 100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing Myeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue usingMyeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue usingMyeloperoxidase (MPO) Rabbit mAb at a dilution of 1:6000 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IF-P
Confocal imaging of paraffin-embedded Ratspleen tissue using Myeloperoxidase (MPO)Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embeddedMouse spleen tissue using Myeloperoxidase(MPO) Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embeddedHuman colon tissue using Myeloperoxidase(MPO) Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:3000-1:10000 ICC/IF:1:50-1:200 IHC:1:5000-1:20000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
