WB
Western blot analysis of lysates from HeLa cells, using THRAP3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.| Product Name | THRAP3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | THRAP3 |
|---|---|
| Gene Synonyms | BCLAF2 TRAP150 |
| Gene Full Name | thyroid hormone receptor associated protein 3 |
| Gene Summary | Enables nuclear thyroid hormone receptor binding activity; phosphoprotein binding activity; and transcription coactivator activity. Involved in positive regulation of circadian rhythm and regulation of gene expression. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in exon-exon junction complex and nuclear speck. Part of mediator complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | BCLAF2 TRAP150 THRAP3 |
| Molecular Weight(MW) | 109kDa(Observed band size:150kDa) |
| Cellular Localization | Nucleus, Nucleus speckle, nucleoplasm. |
WB
Western blot analysis of lysates from HeLa cells, using THRAP3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Human brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain tissue using THRAP3Rabbit pAb at a dilution of 1:100(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
