WB
Western blot analysis of lysates from HeLa cells, using Monoamine Oxidase B (MAOB) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Monoamine Oxidase B (MAOB)Rabbit pAb at dilution of 1:100 (40xlens). Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of L929 cellsusing Monoamine Oxidase B (MonoamineOxidase B (MAOB)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cellsusing Monoamine Oxidase B (MonoamineOxidase B (MAOB)) Rabbit pAb atdilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using MonoamineOxidase B (MAOB) Rabbit pAb atdilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using MonoamineOxidase B (MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.| Product Name | Monoamine Oxidase B (MAOB) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAOB |
|---|---|
| Gene Full Name | monoamine oxidase B |
| Gene Summary | The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq, Jul 2008] |
| Alternative Names | MAOB Monoamine Oxidase B (MAOB) |
| Molecular Weight(MW) | 59kDa(Observed band size:60kDa) |
| Cellular Localization | Cytoplasmic side, Mitochondrion outer membrane, Single-pass type IV membrane protein. |
WB
Western blot analysis of lysates from HeLa cells, using Monoamine Oxidase B (MAOB) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using Monoamine Oxidase B (MAOB)Rabbit pAb at dilution of 1:100 (40xlens). Secondary antibody: Cy3-conjugatedGoat anti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of L929 cellsusing Monoamine Oxidase B (MonoamineOxidase B (MAOB)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cellsusing Monoamine Oxidase B (MonoamineOxidase B (MAOB)) Rabbit pAb atdilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using MonoamineOxidase B (MAOB) Rabbit pAb atdilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using MonoamineOxidase B (MAOB) Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
