WB
Western blot analysis of lysates from Mouse kidney, using ETS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.ICC/IF
Immunofluorescence analysis of A549 cellsusing ETS1 Rabbit pAb. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L)at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of HeLa cellsusing ETS1 Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using ETS1Rabbit pAb at dilution of 1:100 (40xlens). Microwave antigen retrieval performedwith 0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Product Name | ETS1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500- 1:1000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ETS1 |
|---|---|
| Gene Synonyms | p54 ETS-1 EWSR2 c-ets-1 |
| Gene Full Name | ETS proto-oncogene 1, transcription factor |
| Gene Summary | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| Alternative Names | p54 ETS-1 EWSR2 c-ets-1 ETS1 |
| Molecular Weight(MW) | 50kDa(Observed band size:52kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from Mouse kidney, using ETS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
ICC/IF
Immunofluorescence analysis of A549 cellsusing ETS1 Rabbit pAb. Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L)at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing ETS1 Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using ETS1Rabbit pAb at dilution of 1:100 (40xlens). Microwave antigen retrieval performedwith 0.01M PBS Buffer (pH 7.2) prior to IHC staining.| Application Notes | WB:1:500- 1:1000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
